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Development of Antibacterial Photodynamic Periodontal Therapy for Lasers using "Biological Window" of Optical Properties

Development of periodontal treatment using laser and photosensitizer

40

1.Subjects with periodontitis 40 cases(Male 21, female 19)

All subjects completed the experiment.

No discomfort, pain and illness was observed throughout the period.

Primary outcome 1.Change in viable colony counts in periodontal pocket before and immediately after treatment in experimental group was significantly observed. Secondary outcome 2.Significant change in viable colony counts in periodontal pocket before and immediately after treatment in control group was not observed. 3.A significant difference was observed in the comparison (change amount and rate of change) of the viable CFU counts before and immediately after the treatment. 4.A significant difference was not observed in the comparison (change amount and rate of change) of the viable CFU counts before and one week after the treatment. 5.A significant difference was not observed in the comparison (change amount and rate of change) of the CFU counts of realtime PCR before and one week after the treatment. 6.A significant difference was not observed in the comparison (change amount and rate of change) of the percentage of red complex before and one week after the treatment. 7.Significant differences were observed in the pre- and post-treatment chi-square tests (threshold of reduction; 50% and 10%) between the control and experimental groups. However, the 1% threshold was not significant. 8.Np change was observed of periodontal pocket depth in all group. 9.The ratio of Gram(positive) bacteria to Gram(negative) bacteria was significant in the comparison between the groups before and 1 week after treatment, and at 1 week after treatment, the ratio of Gram-negative bacteria was significantly reduced in the experimental group.

In the new a- PDT (antibacterial photodynamic therapy by irradiation from outside the periodontal pocket), a decrease in the viable CFU count immediately after treatment was observed under the conditions obtained in the basic study, However, the effect was not recognized but one week later. It is necessary to revise the irradiation conditions. However, the ratio of Gram(-) bacteria was significantly reduced in the experimental group, indicating that the bacterial flor

Mar. 14, 2022

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https://jrct.mhlw.go.jp/latest-detail/jRCTs041200061

Fukuda Mitsuo

Aichi Gakuin University Dental Hospital

2-11 Suemori-Dori, Chikusa-ku, Nagoya, Aichi

fukuda-m@dpc.agu.ac.jp

Hayashi Jun-ichiro

Aichi Gakuin University Dental Hospital

2-11 Suemori-Dori, Chikua-ku, Nagoya, Aichi.

+81-52-759-2150

jun1row@dpc.agu.ac.jp

Complete

Nov. 10, 2020

Interventional

randomized controlled trial

single blind

placebo control

parallel assignment

treatment purpose

(1) Healthy adults with periodontal disease as follows,
1) who have single-root teeth with over 5mm residual pockets depth.
2) who have stable occulusion.
(2) Patients aged from 20 to 85 years old at registration.
(3) Patients who have written informed content to participate in this study for 2 consecutive weeks.

(1) Patients who have taken antibiotics within the last 3 months.
(2) Patients with diabetes.
(3) Patients who have taken or taking anticancer drugs within 6 months.
(4) Patients with a history of allergy to indocyanine green.
(5) Patients with a history of allergy to crustaceans.
(6) Women who are pregnant, breastfeeding or may or may be pregnant.

Both

Periodontal patients with residual pockets at SPT stage

Experimental group: inject 0.2ml Mano particle(10mg/ml) solution into periodontal pockets with syringe connected of 30G blunt needle.
Contorol group: inject 0.2ml sterile distilled water into periodontal pockets with syringe connected of 30G blunt needle.

Periodontitis

Reduction of live bacteria number immediately after procedure.

(1) Reduction of live bacteria number one week after procedure
(2) Change of bacterial flora one week after procedure
(3) Change of peiodontal pocket depth after procedure

+81-52-858-7215

Oct. 27, 2020

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